Journal: bioRxiv

Article Title: Gut bacteria gatekeep host-specific colonization of a commensal fungus that boosts type 2 immunity

doi: 10.1101/2025.04.30.651489

Figure Lengend Snippet: (A) The heat map of expression of the tuft cell and the goblet cell marker genes in the small intestine ( n = 4 per group). (B) Representative image and the number of tuft cells in the small intestine of control and K. pintolopesii -colonized mice. Tissues were stained with DAPI (nuclei; blue) and DCLK1 (tuft cells; green). 1-7 images were used for tuft cell counting per mouse with 4–6 mice per group. (C) Representative image and the number of goblet cells in the small intestine of control and K. pintolopesii -colonized mice. Tissues were stained with hematoxylin (nuclei) and alcian blue (goblet cells). 6-15 villi were chosen for goblet cell counting per mouse with 4–5 mice per group. (D) Representative flow cytometry plots and frequency of Th2 in the small intestinal lamina propria of control and K. pintolopesii -colonized mice ( n = 10 per group). Th2 populations were gated as live CD45 + Lin + CD90.2 + CD4 + GATA3 + Foxp3 - ST2 + . (E) Fungal burden in the feces of BALB/c mice after K. pintolopesii colonization ( n = 4 per group). (F) Frequency of Th2 in the small intestinal lamina propria of control and K. pintolopesii -colonized BALB/c mice ( n = 4 per group). (G) Scheme of the H. polygyrus challenge. (H) The quantification of worm eggs in the feces of the control and K. pintolopesii -colonized mice (Control, n = 9; Kp, n = 10). Bars indicate mean ± SEM. Significances were determined by the unpaired two-tailed t-test or Mann-Whitney test. *p < 0.05, **p < 0.01, and ****p < 0.0001. Kp, K. pintolopesii -colonized mice.

Article Snippet: For goblet cells, slides were incubated with Alcian blue (StatLab) for 30 min followed by washing in running tap water for 2 min.

Techniques: Expressing, Marker, Control, Staining, Cell Counting, Flow Cytometry, Two Tailed Test, MANN-WHITNEY

Journal: Molecular cancer research : MCR

Article Title: Caspase-9b drives cellular transformation, lung inflammation, and lung tumorigenesis

doi: 10.1158/1541-7786.MCR-21-0905

Figure Lengend Snippet: Macroscopic (A) and microscopic lung tumors observed in C9b mice over 21m old of age are summarized in B. *: One mouse developed a carcinoid tumor and adenocarcinoma. C. Histological analyses including H&E (a-c), Alcian Blue-PAS (d), and IHC staining with indicated antibodies (e-i) of lung tumors identified adenocarcinoma (a,d, g-i), squamous cell carcinoma (scc, b&e), and carcinoid tumors (c&f). Images are taken at 10x magnification. Inset in b is at 40x magnification. D&E. Gr1+ cells (D, IHC) in the lung per low power field (20x, 3 views per mouse) were counted in control (n=9) and C9b mice with (red, n= 5) or without (n=11) lung tumors (E). Data are means ± SEM. Adjusted p values are determined by ANOVA with Tukey’s multiple comparison test.

Article Snippet: Gomori Trichrome staining (Richard-Allan Scientific) and Alcian blue-PAS staining (Statlab) were performed following manufacturers’ instructions.

Techniques: Immunohistochemistry, Control, Comparison

Journal:

Article Title: The Golgi GDPase of the Fungal Pathogen Candida albicans Affects Morphogenesis, Glycosylation, and Cell Wall Properties

doi: 10.1128/EC.1.3.420-431.2002

Figure Lengend Snippet: Cell surface charge in C. albicans strains. (A) Alcian blue binding assays. Relative dye binding was calculated as the percentage of dye bound compared with results for the parental strain (CAI4). (B) Cell wall phosphate content. Results are average of three independent determinations; bars indicate standard deviations. WT, wild type.

Article Snippet: Alcian blue binding assays were carried out using the method of Odani et al. ( 41 ) with minor modifications.

Techniques: Binding Assay